Intermediate

Part:BBa_K5181009:Design

Designed by: Eeshani Umesh Abhyankar   Group: iGEM24_IISER-TVM   (2024-08-29)


tet promoter with RBS


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 636
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 7
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 730
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 636
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 636
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is replaceable with araBAD promoter_RBS composite part(BBa_K5181006) using appropriate primers as listed below:

Forward primer:5’ ATATGCATATATCTCCTTCTTAAAAGATC 3’

Reverse primer:5’ CGCAAGCTTTTATGACAAC 3’


This modular design will facilitate experimentation with two different promoters for the same genes.

Source

Reference paper:

References